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| 1. |  | ALVES, T.; GEISSLITZ, S. M.; NASCIMENTO, T. P. DO; CARVALHO, C. W. P. de; SCHEERF, K. A.; TAKEITI, C. Y.; FERREIRA, M. S. L. A proteomic-based study to assess the effect of extrusion in the allergenic profile of wheat flours of different technological qualities. In: CONFERÊNCIA INTERNACIONAL DE PROTEÍNAS E COLOIDES ALIMENTARES,9., 2023, Rio de Janeiro. Anais... Campinas, Galoá, 2023. Poster 157774. CIPCA 2023. Colóides alimentares e saúde (peptídeos bioativos, alergenicidade).| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/11/2017 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
PINTO, I. S. B.; FONSECA, I.; BRANDAO, H. de M.; GERN, J. C.; GUIMARAES, A. S.; CARVALHO, W. A.; BRITO, M. A. V. P. e; VICCINI, L. F.; MARTINS, M. F. |
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Afiliação: |
ISABELLA S. B. PINTO, CNPq; ISABELA FONSECA, CNPq; HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL; LYDERSON FACIO VICCINI, UFJF; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
SHORT-COMMUNICATION: Evaluation of perfused bovine udder for gene expression studies in dairy cows. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetic and Molecular Research, v. 16, n. 1, 2017. |
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Páginas: |
7 p. |
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DOI: |
10.4238/gmr16019637 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods. |
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Palavras-Chave: |
Ex-situ model; Mammary gland. |
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Thesaurus NAL: |
genes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167362/1/Cnpgl-2017-ShortComm-GMR-Evaluation.pdf
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Marc: |
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008 2017 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr16019637$2DOI
100 1 $aPINTO, I. S. B.
245 $aSHORT-COMMUNICATION$bEvaluation of perfused bovine udder for gene expression studies in dairy cows.$h[electronic resource]
260 $c2017
300 $a7 p.
520 $aAbstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods.
650 $agenes
653 $aEx-situ model
653 $aMammary gland
700 1 $aFONSECA, I.
700 1 $aBRANDAO, H. de M.
700 1 $aGERN, J. C.
700 1 $aGUIMARAES, A. S.
700 1 $aCARVALHO, W. A.
700 1 $aBRITO, M. A. V. P. e
700 1 $aVICCINI, L. F.
700 1 $aMARTINS, M. F.
773 $tGenetic and Molecular Research$gv. 16, n. 1, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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